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KMID : 0370219840280010021
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Yakhak Hoeji
1984 Volume.28 No. 1 p.21 ~ p.23
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Determination of Taurine in Preparations by Amino Acid Autoanalyzer
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박만기/Park MK
한달수/Han DS
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Abstract
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High performance amino acid analyzing method has been developed for the routine analysis of taurine in preparations. Ion-exchange resin it #2619 Hitachi Custom Ion-Exchange Resin, 2.6 (I.D.) X150 (length)mm was used as column, buffer I, pH 3.3 as mobile phase. The retention time of taurine was 7 minutes. Calibration curve by peak height for standard taurine was linear from 2.5ppm to 25ppm. The reproducibility showed relative standard deviation +/-1.9% when analyzed 10 times for standard solution. The samples could be continuously analyzed without regenerating the resin between samples. Five samples were applied to column every 12 min. and then the resin was regenerated for 30 min. during one analyzing cycle time, 90 min. The automatic amino acid analyzer has made it possible to assay multiple samples in a relatively short period of time using the analytical magnetic program card. The high sensitivity and specificity of the analytical column of the automatic amino acid analyzer permits the routine analysis of taurine in preparations.
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